PAB10251

antibody from Abnova Corporation

Targeting: IRAK4 NY-REN-64

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PAB10251

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10251, RRID:AB_1676575

Product name

IRAK4 polyclonal antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to C-terminus of human IRAK4.

Reactivity

Human

Host

Rabbit

Vial size

100 μg

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family.

Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z
The Journal of biological chemistry 1999 Jul 2;274(27):19403-10

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IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.

Muzio M, Ni J, Feng P, Dixit VM
Science (New York, N.Y.) 1997 Nov 28;278(5343):1612-5

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IRAK: a kinase associated with the interleukin-1 receptor.

Cao Z, Henzel WJ, Gao X
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using IRAK4 polyclonal antibody (Cat # PAB10251) shows detection of IRAK4 in HeLa whole cell lysate. The membrane was probed with the primary antibody dilutedto 1:1000 (lane 1), 1 : 500 (lane 2), and 1 : 250 (lane 3) respectively.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemistry using IRAK4 polyclonal antibody (Cat # PAB10251) shows staining of IRAK4 in HeLa cells.The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1 : 100.

Validation comment

Immunocytochemistry