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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- MAB2538 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2538, RRID:AB_10555313
- Product name
- IRAK4 (phospho T345) monoclonal antibody, clone A8A8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic phosphopeptide of IRAK4.
- Antigen sequence
VMTSR- Isotype
- IgG
- Antibody clone number
- A8A8
- Storage
- Store at -20°C to -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration.
Augmentation of therapeutic responses in melanoma by inhibition of IRAK-1,-4.
IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase.
Lehmann SM, Krüger C, Park B, Derkow K, Rosenberger K, Baumgart J, Trimbuch T, Eom G, Hinz M, Kaul D, Habbel P, Kälin R, Franzoni E, Rybak A, Nguyen D, Veh R, Ninnemann O, Peters O, Nitsch R, Heppner FL, Golenbock D, Schott E, Ploegh HL, Wulczyn FG, Lehnardt S
Nature neuroscience 2012 Jun;15(6):827-35
Nature neuroscience 2012 Jun;15(6):827-35
Augmentation of therapeutic responses in melanoma by inhibition of IRAK-1,-4.
Srivastava R, Geng D, Liu Y, Zheng L, Li Z, Joseph MA, McKenna C, Bansal N, Ochoa A, Davila E
Cancer research 2012 Dec 1;72(23):6209-16
Cancer research 2012 Dec 1;72(23):6209-16
IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase.
Li S, Strelow A, Fontana EJ, Wesche H
Proceedings of the National Academy of Sciences of the United States of America 2002 Apr 16;99(8):5567-72
Proceedings of the National Academy of Sciences of the United States of America 2002 Apr 16;99(8):5567-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The 5 x 107 Jurkat cells were stimulated by 50 ng/mL IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with Phosphospecific peptide. Lane 3 was pre-incubate with non-phospho specific peptide.An immunoreactive band is observed around ~51 KDa.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptide was blot onto NC membrane.A : IRAK4 (pT345). B : IRAK4 (non phosphorylated). Followed by IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) incubation at a 1 : 1000 dilution.
- Validation comment
- Dot Blot (Peptide)
