Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29699 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRAK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299. Predicted reactivity: Mouse (89%), Rat (90%), Pig (95%), Sheep (93%), Rhesus Monkey (100%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.54 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IRAK4 Polyclonal Antibody (Product # PA5-29699). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. IRAK4 Polyclonal Antibody (Product # PA5-29699) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of IRAK4 was achieved by transfecting THP-1 with IRAK4 specific siRNAs (Silencer® select Product # s535019, s535020). Western blot analysis (Fig. a) was performed using Whole Cell Extract from the IRAK4 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with IRAK4 Polyclonal Antibody (Product # PA5-29699, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to IRAK4.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IRAK4 Polyclonal Antibody (Product # PA5-29699) and a 52 kDa band corresponding to IRAK4 was observed across cell lines tested. Whole Cell Extract (40 µg lysate) of THP-1 (Lane 1), THP-1 treated with PMA (25 nM for 48 hours) and LPS (100 ng/ml for 24 hours) (Lane 2), THP-1 (Lane 3), THP-1 treated with LPS (100 ng/ml for 12 hours) after M0 macrophage differentiation (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). THP-1 differentiated to macrophage are reported to show down regulation in IRAK4 levels (doi:10.1016/j.cellsig.2008.10.009).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IRAK4 Polyclonal Antibody detects IRAK4 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: IRAK4 Polyclonal Antibody (Product # PA5-29699) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IRAK4 Polyclonal Antibody detects IRAK4 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded human gastric cancer. IRAK4 Polyclonal Antibody (Product # PA5-29699) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.