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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA1-29159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Recommended positive controls: Jurkat Cells, Stomach.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Caspase-3 and caspase-8 expression in breast cancer: caspase-3 is associated with survival.
T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis.
Pu X, Storr SJ, Zhang Y, Rakha EA, Green AR, Ellis IO, Martin SG
Apoptosis : an international journal on programmed cell death 2017 Mar;22(3):357-368
Apoptosis : an international journal on programmed cell death 2017 Mar;22(3):357-368
T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis.
Martin BN, Wang C, Zhang CJ, Kang Z, Gulen MF, Zepp JA, Zhao J, Bian G, Do JS, Min B, Pavicic PG Jr, El-Sanadi C, Fox PL, Akitsu A, Iwakura Y, Sarkar A, Wewers MD, Kaiser WJ, Mocarski ES, Rothenberg ME, Hise AG, Dubyak GR, Ransohoff RM, Li X
Nature immunology 2016 May;17(5):583-92
Nature immunology 2016 May;17(5):583-92
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 8 was performed by loading 30 µg of THP-1 whole cell lysate per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Caspase 8 polyclonal antibody (Product # PA1-29159) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 8 was performed by loading 30 µg of THP-1 whole cell lysate per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Caspase 8 polyclonal antibody (Product # PA1-29159) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Caspase 8 (green) HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Caspase 8 polyclonal antibody (Product # PA1-29159) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat-anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
