sc-5559

antibody from Santa Cruz Biotechnology

Targeting: FADD GIG3, MORT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-5559

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-5559, RRID:AB_2100622

Product name

Anti-FADD

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation.

Huang Y, Chen L, Zhou Y, Liu H, Yang J, Liu Z, Wang C
Molecular biology of the cell 2011 Apr 15;22(8):1389-97

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Detection of epithelial apoptosis in pelvic ileal pouches for ulcerative colitis and familial adenomatous polyposis.

Leal RF, Ayrizono Mde L, Milanski M, Fagundes JJ, Moraes JC, Meirelles LR, Velloso LA, Coy CS
Journal of translational medicine 2010 Jan 29;8:11

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High-fat diet induces apoptosis of hypothalamic neurons.

Moraes JC, Coope A, Morari J, Cintra DE, Roman EA, Pauli JR, Romanatto T, Carvalheira JB, Oliveira AL, Saad MJ, Velloso LA
PloS one 2009;4(4):e5045

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A new caspase-8 isoform caspase-8s increased sensitivity to apoptosis in Jurkat cells.

Xu Z, Tang K, Wang M, Rao Q, Liu B, Wang J
Journal of biomedicine & biotechnology 2009;2009:930462

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Effect of cocaine on Fas-associated protein with death domain in the rat brain: individual differences in a model of differential vulnerability to drug abuse.

García-Fuster MJ, Clinton SM, Watson SJ, Akil H
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2009 Apr;34(5):1123-34

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Dynamics of RASSF1A/MOAP-1 association with death receptors.

Foley CJ, Freedman H, Choo SL, Onyskiw C, Fu NY, Yu VC, Tuszynski J, Pratt JC, Baksh S
Molecular and cellular biology 2008 Jul;28(14):4520-35

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K1 protein of human herpesvirus 8 suppresses lymphoma cell Fas-mediated apoptosis.

Wang S, Wang S, Maeng H, Young DP, Prakash O, Fayad LE, Younes A, Samaniego F
Blood 2007 Mar 1;109(5):2174-82

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Effects of Opiate Drugs on Fas-Associated Protein with Death Domain (FADD) and Effector Caspases in the Rat Brain: Regulation by the ERK1/2 MAP Kinase Pathway

María-Julia García-Fuster, Antonio Miralles, Jesús A García-Sevilla
Neuropsychopharmacology 2006 Feb;32(2):399-411

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 229, 107, 78, 46, 31, 26, 16.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 23

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.