MA1-20168

antibody from Invitrogen Antibodies

Targeting: FADD GIG3, MORT1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-20168

Provider

Invitrogen Antibodies

Product name

FADD Monoclonal Antibody (FD19)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: A431. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

FD19

Vial size

100 µL

Concentration

2.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Induction of interferon-β and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells.

Granqvist V, Holmgren C, Larsson C
PloS one 2021;16(3):e0248175

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 6 Involvement of FADD in TRAIL-mediated IFN signaling. A-D) MCF-7 cells were stimulated with 100 ng/ml TRAIL for 2 h in the presence of 20 muM zVAD-FMK and lysates were immunoprecipitated with antibodies towards FADD (A, B) or caspase-8 (C, D). Lysates and immunoprecipitates were subjected to Western blot for FADD and caspase-8 (A and C). Intensities in bands for the immunoprecipitates were quantified (B and D). E-G) MCF-7 cells were transfected for 72 h with a combination of two siRNAs targeting FADD (E) followed by treatment with TRAIL (100 ng/ml) for 48h. Lysates were subjected to Western blot with indicated antibodies (F) and intensities in bands were quantified (G). Data (B,D,G) are mean +- SEM with individual data points indicated and experiments were performed three (B) or four (D,G) times. * denotes p