H00008772-D01P

antibody from Abnova Corporation

Targeting: FADD GIG3, MORT1

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008772-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008772-D01P, RRID:AB_1573473

Product name

FADD purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human FADD protein.

Antigen sequence

MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRK
LERVQSGLDLFSMLLEQNDLEPGHTELLRELLASL
RRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVIC
DNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTER
VRESLRIWKNTEKENATVAHLVGALRSCQMNLVAD
LVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FADD expression in transfected 293T cell line (H00008772-T01) by FADD MaxPab polyclonal antibody.Lane 1: FADD transfected lysate(23.30 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

FADD MaxPab rabbit polyclonal antibody. Western Blot analysis of FADD expression in human kidney.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FADD and BID. HeLa cells were stained with anti-FADD rabbit purified polyclonal 1:1200 and anti-BID mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)