Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- RQ6175 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Fadd Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific Fadd antibody is suitable for use in Western blot/Immunohistochemistry applications with rat, mouse samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Fadd antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) rat thymus, 3) rat PC-12, 4) mouse brain, 5) mouse thymus and 6) mouse NIH 3T3 lysate with Fadd antibody. Predicted molecular weight ~23 kDa, commonly observed at 23-28 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat liver cells with Fadd antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.