HPA001464

antibody from Atlas Antibodies

Targeting: FADD GIG3, MORT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA001464

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001464, RRID:AB_1848348

Product name

Anti-FADD

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQND
LEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAG
AAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDT
KIDSIEDRYPRNLTERVRESLRIWKN


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines A-431 and HeLa using Anti-FADD antibody. Corresponding FADD RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line Jurkat.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & the Golgi apparatus.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human gallbladder shows cytoplasmic positivity in glandular cells.

Sample type

HUMAN