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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF2938 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FADD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human FADD in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13158- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells.
English WR, Ireland-Zecchini H, Baker AH, Littlewood TD, Bennett MR, Murphy G
PloS one 2018;13(4):e0195116
PloS one 2018;13(4):e0195116
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human FADD by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human FADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FADD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
