NBP1-77775-0.1mg

antibody from Novus Biologicals

Targeting: ADA ADA1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-77775-0.1mg

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Adenosine Deaminase/ADA Antibody

Antibody type

Polyclonal

Description

Ion exchange chromatography.

Reactivity

Bovine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

LYOPH

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Lane 1: Adenosine Deaminase (Calf Spleen). Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.