PAB28429

antibody from Abnova Corporation

Targeting: ADA ADA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28429

Provider

Abnova Corporation

Product name

ADA polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ADA.

Antigen sequence

GDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEV
GSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLR
QENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQA
NYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ADA polyclonal antibody (Cat # PAB28429) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U-2 OS with ADA polyclonal antibody (Cat # PAB28429) at 1-4 ug/mL dilution shows positivity in plasma membrane & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with ADA polyclonal antibody (Cat # PAB28429) shows distinct cytoplasmic and nuclear positivity in germinal center cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)