GTX628259

ADA antibody from GeneTex

ADA1

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunoprecipitation

Supportive data in Antibodypedia

Provider product page for GTX628259

Antibody Data

Product number

GTX628259

Provider

GeneTex

Product name

ADA antibody [EPR4437]

Provider product page

Antibody type

Monoclonal

Host

Rabbit

Vial size

100 µl

References

Sororin loads to the synaptonemal complex central region independently of meiotic cohesin complexes.
Gómez R, Felipe-Medina N, Ruiz-Torres M, Berenguer I, Viera A, Pérez S, Barbero JL, Llano E, Fukuda T, Alsheimer M, Pendás AM, Losada A, Suja JA
EMBO reports 2016 May;17(5):695-707

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Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity.
Lin W, Sampathi S, Dai H, Liu C, Zhou M, Hu J, Huang Q, Campbell J, Shin-Ya K, Zheng L, Chai W, Shen B
The EMBO journal 2013 May 15;32(10):1425-39

Western blot

Supportive validation

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GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Non-transfected HeLa lysates B: LGALS4-myc transfected HeLa lysates 10% SDS PAGE GTX628259 diluted at 1:5000

Validation comment

WB

Supportive validation

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GeneTex (provider)

Main image

Experimental details

Myc-tag antibody detects Myc-tag protein by western blot analysis. Non-transfected (-) and Myc-tagged protein-transfected (+), 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Myc-tag antibody (GTX628259) diluted by 1:20000.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Myc tag antibody [GT0002] detects Myc-recombinant protein by immunofluorescent analysis. Sample: Myc tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Myc-recombinant protein stained by Myc tag antibody [GT0002] (GTX628259) diluted at 1:2000. Blue: Hoechst 33342 staining.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Myc tag antibody [GT0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [GT0002] (GTX628259). (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected by GTX628259 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX221667-01) was used in Western blot.