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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX628259 - Provider product page
- Provider
- GeneTex
- Product name
- ADA antibody [EPR4437]
- Antibody type
- Monoclonal
- Host
- Rabbit
- Vial size
- 100 µl
Submitted references Sororin loads to the synaptonemal complex central region independently of meiotic cohesin complexes.
Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity.
Gómez R, Felipe-Medina N, Ruiz-Torres M, Berenguer I, Viera A, Pérez S, Barbero JL, Llano E, Fukuda T, Alsheimer M, Pendás AM, Losada A, Suja JA
EMBO reports 2016 May;17(5):695-707
EMBO reports 2016 May;17(5):695-707
Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity.
Lin W, Sampathi S, Dai H, Liu C, Zhou M, Hu J, Huang Q, Campbell J, Shin-Ya K, Zheng L, Chai W, Shen B
The EMBO journal 2013 May 15;32(10):1425-39
The EMBO journal 2013 May 15;32(10):1425-39
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Non-transfected HeLa lysates B: LGALS4-myc transfected HeLa lysates 10% SDS PAGE GTX628259 diluted at 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Myc-tag antibody detects Myc-tag protein by western blot analysis. Non-transfected (-) and Myc-tagged protein-transfected (+), 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Myc-tag antibody (GTX628259) diluted by 1:20000.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Myc tag antibody [GT0002] detects Myc-recombinant protein by immunofluorescent analysis. Sample: Myc tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Myc-recombinant protein stained by Myc tag antibody [GT0002] (GTX628259) diluted at 1:2000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Myc tag antibody [GT0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [GT0002] (GTX628259). (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected by GTX628259 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX221667-01) was used in Western blot.
