F41596

antibody from NSJ Bioreagents

Targeting: ADA ADA1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F41596

Provider

NSJ Bioreagents

Product name

ADA Antibody

Antibody type

Polyclonal

Description

This highly specific ADA antibody is suitable for use in Flow cytometry/Western blot/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the ADA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ADA antibody western blot analysis in HL-60 lysate

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) MOLT-4, 2) Jurkat, 3) HeLa, 4) HL-60 and 5) DU145 cell lysate with ADA antibody. Expected molecular weight ~41 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ADA antibody western blot analysis in HL-60 lysate. Expected molecular weight ~41 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human duodenum tissue with ADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ADA antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ADA antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.