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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- R32414 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ADA Antibody / Adenosine deaminase
- Antibody type
- Polyclonal
- Antigen
- Amino acids Q135-L363 of the human protein were used as the immunogen for the Adenosine deaminase antibody.
- Description
- Antigen affinity
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Adenosine deaminase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) COLO320 cell lysate with Adenosine deaminase antibody. Expected/observed molecular weight ~41 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) rat thymus and 3) mouse thymus tissue lysate with Adenosine deaminase antibody. Expected molecular weight ~41 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with Adenosine deaminase antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human intestinal cancer tissue with Adenosine deaminase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human Jurkat cells with Adenosine deaminase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Adenosine deaminase antibody.
