PB9914

ADA antibody from Boster Biological Technology

ADA1

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Immunohistochemistry

Data presented on provider website

Flow cytometry

Data presented on provider website

Provider product page for PB9914

Antibody Data

Product number

PB9914

Provider

Boster Biological Technology

Product name

Anti-ADA Antibody Picoband™

Provider product page

Antibody type

Polyclonal

Description

Polyclonal antibody for Adenosine Deaminase/ADA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human. Adenosine Deaminase/ADA information: Molecular Weight: 40764 MW; Subcellular Localization: Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen . Cytoplasm . Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion; Tissue Specificity: Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of ADA/Adenosine Deaminase using anti- ADA/Adenosine Deaminase antibody (PB9914). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ADA/Adenosine Deaminase antigen affinity purified polyclonal antibody (Catalog # PB9914) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADA/Adenosine Deaminase at approximately 41KD. The expected band size for ADA/Adenosine Deaminase is at 41KD.

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