H00000100-M01

antibody from Abnova Corporation

Targeting: ADA ADA1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000100-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000100-M01, RRID:AB_425288

Product name

ADA monoclonal antibody (M01), clone 4G4-1C6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ADA.

Antigen sequence

MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRG
IALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMP
AIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPH
LLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEG
ERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQ
TVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIH
RTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLED
QALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAV
IRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDM
GFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKA
YGMPPSASAGQNL


Isotype

IgG

Antibody clone number

4G4-1C6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ADA monoclonal antibody (M01), clone 4G4-1C6. Western Blot analysis of ADA expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ADA monoclonal antibody (M01), clone 4G4-1C6. Western Blot analysis of ADA expression in HL-60 ( Cat # L014V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ADA is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol