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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- MA5-24586 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenosine Deaminase Monoclonal Antibody (CL0176)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SDNQPEGMIS ESLDNLESMM PNKVRKIGEL VRYLNTNPVG GLLEYARSHG FAAEFKLVDQ SGPPHEPKFV YQAKVGGRWF PAVCAHSKKQ GKQEAADAAL RVLIGENEKA ERMGFTEVTP VTGASLRRTM LLLSRSPEA Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 85%.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0176
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Adenosine Deaminase in Lane1: Marker (kDa), Lane 2: Human cell line U-251 MG Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Adenosine Deaminase Monoclonal Antibody (CL0176) (Product # MA5-24586). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ADA in A549 cells using Adenosine Deaminase Monoclonal Antibody (CL0176) (Product # MA5-24586), showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human testis tissue shows nuclear positivity. Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human kidney tissue shows nuclear positivity. Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human cerebral cortex tissue shows nuclear positivity. Samples were probed using a Adenosine Deaminase Monoclonal Antibody (Product # MA5-24586).
