Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-C745033 - Provider product page
- Provider
- LSBio
- Product name
- ADA / Adenosine Deaminase Antibody (Lyophilized, HRP) LS-C745033
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Bovine
- Host
- Rabbit
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated. Lane 1: Adenosine Deaminase (Calf Spleen). Load: 50 ng per lane. Primary antibody: Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 41 kDa, 45 kDa for Adenosine Deaminase (Calf Spleen).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Biotin conjugated anti adenosine deaminase was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.