PA5-80316

antibody from Invitrogen Antibodies

Targeting: ADA ADA1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80316

Provider

Invitrogen Antibodies

Product name

Adenosine Deaminase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody has specificity for Human Adenosine Deaminase/ADA.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Adenosine Deaminase in Lane A: Jurkat Whole Cell Lysate (30 µg). Samples were probed using a Adenosine Deaminase Polyclonal Antibody (Product # PA5-80316) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), HRP Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:41 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Adenosine Deaminase Polyclonal Antibody (Product # PA5-80316) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Adenosine Deaminase in human brain with Adenosine Deaminase Polyclonal Antibody (Product # PA5-80316, 1:2,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Adenosine Deaminase in human duodenum with Adenosine Deaminase Polyclonal Antibody (Product # PA5-80316, 1:2,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Adenosine Deaminase Immunoprecipitation using: Lane A: 0.5 mg mouse brain tissue lysate 4 µL with Adenosine Deaminase Polyclonal Antibody (Product # PA5-80316) and 60 μg of Immunomagnetic beads Protein A/G.Primary antibody: Adenosine Deaminase Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 28 kDa.