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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- MA5-33006 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenosine Deaminase Monoclonal Antibody (6D4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6D4
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Adenosine Deaminase in Lane 1: human HeLa whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) using a 0.5 µg/mL dilution, followed by a goat anti-mouse IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human rectal cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Adenosine Deaminase in paraffin-embedded human tonsil tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a 1 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Adenosine Deaminase in U20S cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Adenosine Deaminase monoclonal antibody (Product # MA5-33006) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-mouse IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.
