AF7048

antibody from R&D Systems

Targeting: ADA ADA1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7048

Provider

R&D Systems

Product name

Human/Mouse Adenosine Deaminase/ADA Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human and mouse Adenosine Deaminase/ADA in Western blots.

Reactivity

Human, Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P00813


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human Adenosine Deaminase/ADA Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Adenosine Deaminase/ADA knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Adenosine Deaminase/ADA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7048) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosine Deaminase/ADA at approximately 45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Adenosine Deaminase/ADA by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Adenosine Deaminase/ADA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7048) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosine Deaminase/ ADA at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.