Antibody data
- Product number
- AF7048
- Provider
- R&D Systems
- Product name
- Human/Mouse Adenosine Deaminase/ADA Antibody
- Provider product page
- R&D Systems - AF7048
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Adenosine Deaminase/ADA in Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P00813
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Supportive validation
- Submitted by
-
R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human Adenosine Deaminase/ADA Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Adenosine Deaminase/ADA knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Adenosine Deaminase/ADA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7048) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosine Deaminase/ADA at approximately 45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line.
Supportive validation
- Submitted by
-
R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Adenosine Deaminase/ADA by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Adenosine Deaminase/ADA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7048) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosine Deaminase/ ADA at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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