HPA002022

antibody from Atlas Antibodies

Targeting: WAS IMD2, THC, WASP, WASPA

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA002022

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA002022, RRID:AB_1080581

Product name

Anti-WAS

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQA
GLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQL
PPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLS
LGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSG
KK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human appendix and kidney tissues using Anti-WAS antibody. Corresponding WAS RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp and cells in white pulp.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows low expression as expected.

Sample type

HUMAN