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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-52244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLYYQTEKYD LAIKDLKEAL IQLRGNQLID YKILGLQFKL FACEVLYNIA FMYAKKEEWK KAEEQLALAT SMKSEPRHSK IDKAMECVWK QKLYEPVVIP VGRLFRPNER QVAQLAKKDY LGKATVVASV VDQDSFSGFA PLQPQAA
- Concentration
- 0.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCF2 in Lane 1: Marker (kDa) 229, 112, 84, 48, 32, 27, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted). Samples were probed using a NCF2 Polyclonal Antibody (Product # PA5-52244).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NCF2 in human cell line A-431 shows positivity in cytoplasm. Samples were probed using a NCF2 Polyclonal Antibody (Product # PA5-52244).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NCF2 in human cell line U-251 MG using a NCF2 Polyclonal Antibody (Product # PA5-52244) shows localization to nucleoli and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCF2 in human bone marrow using NCF2 Polyclonal Antibody (Product # PA5-52244) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCF2 in human skeletal muscle using NCF2 Polyclonal Antibody (Product # PA5-52244) shows low expression as expected.
