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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-51702 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QEESSPQSDI PAPPSSKAPG RPQLSPGQKQ KEEPKEVKLS VPMPYTLKVH YKYTVVMKTQ PGLPYSQVRD MVSKKLELRL EHTKLSYRPR DSNELVPLSE DSMKDAWGQV KNYCLTLWCE NTVGDQGFPD EPKESEKADA NNQTTEPQ
- Concentration
- 0.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCF2 in Lane 1: Marker (kDa) 206, 113, 82, 49, 32, 26, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human cell line A-431; Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a NCF2 Polyclonal Antibody (Product # PA5-51702).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-NCF2 Polyclonal Antibody (Product # PA5-51702) and a 60 kDa band corresponding to NCF2 was observed in THP-1 but not in A-431 and HeLa which are reported to be negative. An uncharacterized band (*) around 40 kDa was observed in all the cell lines tested. Whole cell extracts (1%SDS) (30 µg lysate) of THP-1 (Lane 1), A-431 (Lane 2) and HeLa (Lane 3) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCF2 in human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells. Samples were probed using a NCF2 Polyclonal Antibody (Product # PA5-51702).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCF2 in human bone marrow and skeletal muscle tissues using NCF2 Polyclonal Antibody (Product # PA5-51702). Corresponding NCF2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCF2 in human skeletal muscle using NCF2 Polyclonal Antibody (Product # PA5-51702) shows low expression as expected.
