HPA002327

antibody from Atlas Antibodies

Targeting: NCF2 NOXA2, p67phox

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA002327

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA002327, RRID:AB_1079550

Product name

Anti-NCF2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

QEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPK
EVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRD
MVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKD
AWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADA
NNQTTEPQ


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human cell line A-431Lane 5: Human liver tissueLane 6: Human tonsil tissue

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human bone marrow and skeletal muscle tissues using Anti-NCF2 antibody. Corresponding NCF2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN