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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-27843 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENY2 Monoclonal Antibody (GT245)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U87-MG, SK-N-SH, IMR32 Predicted Reactivity: Mouse (100%), Zebrafish (85%), Xenopus laevis (93%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT245
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENY2 in A) U87-MG whole cell lysate, B) SK-N-SH whole cell lysate, C) IMR32 whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with ENY2 monoclonal antibody (Product # MA5-27843) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843). Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ENY2 antibody detects ENY2 protein at Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: ENY2 protein stained by ENY2 antibody (Product # MA5-27843) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ENY2 Monoclonal Antibody (GT245) detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
