PA3-16527

antibody from Invitrogen Antibodies

Targeting: MRE11 ATLD, MRE11A

Antibody data

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Validation data

Product number: PA3-16527 - Provider product page
Provider: Invitrogen Antibodies
Product name: MRE11 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: This Mre11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections (PMID 21279473) Immunoprecipitation and Western Blot.
Concentration: Conc. Not Determined

MRE11 protein structure - PA3-16527 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Mre11 on 50 µg of HeLa and MEF lysates, using Product # PA3-16527

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MRE11 in HeLa and MEF lysates. Samples were incubated in MRE11 polyclonal antibody (Product # PA3-16527 using a dilution of 50 µg. Displaying bands at the molecular weight of 81 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MRE11 in 0.5 mg/mL HeLa lysate. Samples were incubated in MRE11 polyclonal antibody (Product # PA3-16527). A band appears at the theoretical molecular weight of 81 kDa for this product. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of MRE11 was achieved by transfecting HeLa cells with MRE11 specific siRNAs (Silencer® select Product # s8959, s8960). Western blot analysis (Fig. a) was performed using whole cell extracts from the MRE11 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with MRE11 Polyclonal Antibody (Product # PA3-16527, 1:5000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MRE11.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), SKNAS (Lane 2), K562 (Lane 3), Jurkat (Lane 4), Raji (Lane 5), A431 (Lane 6), HepG2 (Lane 7) and IMR-32 (Lane 8). The blot was probed with Anti-MRE11 Polyclonal Antibody (Product # PA3-16527, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). An 81kDa band corresponding to MRE11 was observed across all cell lines tested.