AF2284

antibody from Novus Biologicals

Targeting: MRE11 ATLD, MRE11A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2284

Provider

Novus Biologicals

Product name

Chicken Polyclonal Mre11 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Mre11 in Western blots.

Reactivity

Human

Host

Chicken/Avian

Isotype

IgY

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available.

Rapp A, Greulich KO
Journal of cell science 2004 Oct 1;117(Pt 21):4935-45

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Mre11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Mre11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2284) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for Mre11 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.