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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX111814 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111814, RRID:AB_11167298
- Product name
- Mre11 antibody [C1C3-2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX111814 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mre11 antibody [C1C3-2] detects Mre11 protein at nucleus by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Mre11 protein stained by Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Mre11 protein from HeLa whole cell extracts using 5 £gg of Mre11 antibody [C1C3-2] (GTX111814).Western blot analysis was performed using Mre11 antibody [C1C3-2] (GTX111814).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
