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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319244 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Mre11 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Mre11 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MRE11
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.05 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein, respectively. After SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4?. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.
- Validation comment
- WB
