LS-C18848

antibody from LSBio

Targeting: MRE11 ATLD, MRE11A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C18848

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C18848, RRID:AB_2145251

Product name

MRE11A / MRE11 Antibody LS-C18848

Antibody type

Polyclonal

Description

Affinity purified

Host

Rabbit

Isotype

IgG

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Mre11 (S. cerevisiae) Antibody - Chromatin Immunoprecipitation (ChIP). Chromatin Immunoprecipitation (ChIP) using Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. immune complexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorimeter. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.