Explore
Validate
Learn
Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX12159 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX12159, RRID:AB_625966
- Product name
- Mre11 antibody
- Antibody type
- Polyclonal
- Host
- Rabbit
Submitted references MRE11 function in response to topoisomerase poisons is independent of its function in double-strand break repair in Saccharomyces cerevisiae.
Hamilton NK, Maizels N
PloS one 2010 Oct 28;5(10):e15387
PloS one 2010 Oct 28;5(10):e15387
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) using GeneTex Affinity Purified Mre11 (S. cerevisiae) antibody (GTX12159). A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green? (Invitrogen), and detected using a Fuji scanning fluorometer.
