PAB10276

antibody from Abnova Corporation

Targeting: MRE11 ATLD, MRE11A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10276

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10276, RRID:AB_1676654

Product name

Mre11a polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against partial recombinant Mre11a.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The Mre11/Rad50/Xrs2 complex and non-homologous end-joining of incompatible ends in S. cerevisiae.

Zhang X, Paull TT
DNA repair 2005 Nov 21;4(11):1281-94

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Molecular basis of ataxia telangiectasia and related diseases.

Ball LG, Xiao W
Acta pharmacologica Sinica 2005 Aug;26(8):897-907

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Disruption of mechanisms that prevent rereplication triggers a DNA damage response.

Archambault V, Ikui AE, Drapkin BJ, Cross FR
Molecular and cellular biology 2005 Aug;25(15):6707-21

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using Mre11a polyclonal antibody (Cat # PAB10276) shows detection of a band ~ 80 KDa corresponding to mouse Mre11a (arrowhead).Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11a protein, respectively.After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C.The membrane was then washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.