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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX48735 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48735, RRID:AB_11163494
- Product name
- Mre11 antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse
- Host
- Rabbit
- Storage
- Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing.
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's Affinity Purified anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ?g, 0.3 ?g, 0.1 ?g and 0.05 ?g of purified mouse Mre11 protein, respectively. After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4¢XC. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
