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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Flow cytometry [1]
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- Product number
- NB100-64420 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64420, RRID:AB_960729
- Product name
- Sheep Polyclonal C1q Antibody
- Antibody type
- Polyclonal
- Description
- IgG purified. Recognizes human C1q, a sub-component of the C1 complement component.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 0.5 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: C1q Antibody [NB100-64420] - Effects of SAP on C1q Binding to S. pneumoniae Measured Using Flow Cytometry. (A-C) Proportion of ST2 (A), ST4 (B), and ST23F (C) S. pneumoniae bacteria positive for C1q after incubation in 90 ug/ml human C1q with or without addition of 10 or 50 ug/ml hSAP. (D) Example of flow cytometry histograms of C1q binding to ST4 S. pneumoniae after incubation in 90 ug/ml human C1q with or without addition of 50 ug/ml hSAP. (E) Proportion of S. pneumoniae positive for C1q after incubation in human serum with (black shading) or without (grey shading) addition of 50 ug/ml hSAP, using C1q-depleted serum (white shading) as a negative control. (F) Example of flow cytometry histograms of C1q binding to ST2 S. pneumoniae after incubation in human serum with or without addition of 50 ug/ml hSAP.
