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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-97056 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFA7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MASATRLIQR LRNWASGHDL QGKLQLRYQE ISKRTQPPPK LPVGPSHKLS NNYYCTRDGR RESVPPSIIM SSQKALVSGK PAESSAVAAT EKKAVTPAPP IKRWELSSDQ PYL; Positive Samples: A-549, HepG2, MCF-7, Mouse kidney, Mouse brain, Mouse liver, Mouse heart, Rat liver; Cellular Location: Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.28 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFA7 in extracts of various cell lines. Samples were incubated with NDUFA7 Polyclonal antibody (Product # PA5-97056) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFA7 in extracts from normal (control) and NDUFA7 knockout (KO) 293T cells. Samples were incubated with NDUFA7 Polyclonal antibody (Product # PA5-97056) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
