PAB28388

antibody from Abnova Corporation

Targeting: C1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28388

Provider

Abnova Corporation

Product name

C1R polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C1R.

Antigen sequence

PNNFETTTVITVPTGYRVKLVFQQFDLEPSEGCFY
DYVKISADKKSLGRFCGQLGSPLGNPPGKKEFMSQ
GNKMLLTFHTDFSNEENGTIMFYKGFLAYYQAVDL
DECASRSKSGEEDPQPQCQHLC


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with C1R polyclonal antibody (Cat # PAB28388) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with C1R polyclonal antibody (Cat # PAB28388) at 1-4 ug/ml shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow with C1R polyclonal antibody (Cat # PAB28388) shows cytoplasmic positivity in bone marrow poietic cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)