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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-28028 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1r Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, Raji. Predicted reactivity: Rat (80%), Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C1r using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a C1r polyclonal antibody (Product # PA5-28028) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of C1r was performed by separating 30 µg of Human plasma by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a C1r Polyclonal Antibody (Product # PA5-28028) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C1r was performed by separating 50 µg of rat plasma by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a C1r Polyclonal Antibody (Product # PA5-28028) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C1r in methanol-fixed HCT116 cells using a C1r polyclonal antibody (Product # PA5-28028) at a 1:500 dilution.
