Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 21514-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of GPR50 antibody (21514-1-AP, 1:5,000) by Dr. Qian Li, University of Edinburgh. U1: Lysates from SH-SY5Y cells, untransfected, no endogenous GPR50, negative control,15ug; G1: Lysates from HEK293 cells, with transfected GPR50, positive control, 2ug; G2: Lysates from HEK293 cells, with transfected GPR50, 10ug; U2: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug; U3: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug. Optimal dilution: 1 in 5000, Signal detection: ECL, Pierce; Exposure time: 10min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21514-1-AP (GPR50 antibody) at dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of GPR50 antibody (21514-1-AP, 1:1,000) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of GPR50 antibody (21514-1-AP, 1:1,000) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of GPR50 antibody (21514-1-AP, 1:50) with mouse hippocampus by Dr. Qian Li, University of Edinburgh.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were stained with 0.2ug GPR50 antibody (21514-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
