Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- MA1-91161 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD55 Monoclonal Antibody (BRIC-216)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BRIC-216
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells.
Sartain SE, Turner NA, Moake JL
Journal of immunology (Baltimore, Md. : 1950) 2016 Jan 15;196(2):832-45
Journal of immunology (Baltimore, Md. : 1950) 2016 Jan 15;196(2):832-45
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Complement decay-accelerating factor was performed using 70% confluent log phase HeLa (Positive) and IMR-32 (Negative) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD55 Monoclonal Antibody (BRIC-216) (Product # MA1-91161) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasm (Plasma membrane-like) localization. Panel e represents IMR-32. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Complement decay-accelerating factor was achieved by transfecting HeLa cells with Complement decay-accelerating factor specific siRNA (Silencer® select Product # S534833, S3906). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Complement decay-accelerating factor specific siRNA (panel c,f). Cells were fixed, permeabilized and labelled with CD55 Monoclonal Antibody (BRIC-216) (Product # MA1-91161,1:100 dilution) followed by Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. Significant reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Complement decay-accelerating factor (green). The Images were captured at 60X magnification.