MA1-91161

antibody from Invitrogen Antibodies

Targeting: CD55 CR, CROM, DAF, TC

Western blot

Immunoprecipitation

Flow cytometry

Blocking/Neutralizing

Antibody data

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Validation data

Product number: MA1-91161 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD55 Monoclonal Antibody (BRIC-216)
Antibody type: Monoclonal
Antigen: Other
Description: Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: BRIC-216
Vial size: 200 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CD55 protein structure - MA1-91161 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Complement decay-accelerating factor was performed using 70% confluent log phase HeLa (Positive) and IMR-32 (Negative) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD55 Monoclonal Antibody (BRIC-216) (Product # MA1-91161) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasm (Plasma membrane-like) localization. Panel e represents IMR-32. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Complement decay-accelerating factor was achieved by transfecting HeLa cells with Complement decay-accelerating factor specific siRNA (Silencer® select Product # S534833, S3906). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Complement decay-accelerating factor specific siRNA (panel c,f). Cells were fixed, permeabilized and labelled with CD55 Monoclonal Antibody (BRIC-216) (Product # MA1-91161,1:100 dilution) followed by Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. Significant reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Complement decay-accelerating factor (green). The Images were captured at 60X magnification.