Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- NBP2-47963-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD55/DAF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963] - Using the Alexa Fluor (R) 647 direct conjugate A surface stain was performed on HT-29 cells with CD55/DAF (F4-29D9) antibody NBP2-47963AF647 and a matched isotype control NBP1-97005AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)