H00001604-M02

antibody from Abnova Corporation

Targeting: CD55 CR, CROM, DAF, TC

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001604-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001604-M02, RRID:AB_1111783

Product name

DAF monoclonal antibody (M02), clone 1D7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant DAF.

Antigen sequence

DCGLPPDVPNAQPALEGRTSFPEDTVITYKCEESF
VKIPGEKDSVICLRGSQWSDIEEFCNRSCEVPTRL
NSASLKQPYITQNYFPVGTVVEYECRPGYR


Isotype

IgG

Antibody clone number

1D7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CD55 expression in transfected 293T cell line by DAF monoclonal antibody (M02), clone 1D7.Lane 1: CD55 transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CD55 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CD55 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol