Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 11-0559-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD55 Monoclonal Antibody (143-30), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 143-30 monoclonal antibody reacts with human CD55, the 64-73 kDa molecule also known as DAF (Decay-Accelerating Factor). CD55 is a GPI-anchored single chain glycoprotein expressed on all cells in contact with serum including all hematopoietic cells and vascular endothelium; a soluble form of CD55 is also present in plasma and body fluids. CD55 is thought to play a role in the protection of cells from the damage by autologous complement via prevention of the amplification steps of the complement cascade. Antibodies against CD55 are mitogenic in the presence of phorbol esters. Applications Reported: The 143-30 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 143-30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 143-30
- Vial size
- 25 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human papillomavirus E6 protein enriches the CD55(+) population in cervical cancer cells, promoting radioresistance and cancer aggressiveness.
Leung TH, Tang HW, Siu MK, Chan DW, Chan KK, Cheung AN, Ngan HY
The Journal of pathology 2018 Feb;244(2):151-163
The Journal of pathology 2018 Feb;244(2):151-163
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (open histogram) or FITC Anti-Human CD55 FITC (filled histogram). Total viable cells were used for analysis.
- Conjugate
- Green dye