14-0559-82

antibody from Invitrogen Antibodies

Targeting: CD55 CR, CROM, DAF, TC

Flow cytometry (Recommended by provider)

Antibody Data

Product number

14-0559-82

Provider

Invitrogen Antibodies

Product name

Anti-CD55 Monoclonal Antibody (143-30), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The 143-30 monoclonal antibody reacts with human CD55, the 64-73 kDa molecule also known as DAF (Decay-Accelerating Factor). CD55 is a GPI-anchored single chain glycoprotein expressed on all cells in contact with serum including all hematopoietic cells and vascular endothelium; a soluble form of CD55 is also present in plasma and body fluids. CD55 is thought to play a role in the protection of cells from the damage by autologous complement via prevention of the amplification steps of the complement cascade. Antibodies against CD55 are mitogenic in the presence of phorbol esters. Applications Reported: The 143-30 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 143-30 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

143-30

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C