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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF2009 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD55/DAF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD55/DAF in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08174- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis.
Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Kim HR, Kim KW, Kim BM, Cho ML, Lee SH
PloS one 2015;10(4):e0124909
PloS one 2015;10(4):e0124909
Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism.
Sémiramoth N, Gleizes A, Turbica I, Sandré C, Gorges R, Kansau I, Servin A, Chollet-Martin S
Journal of leukocyte biology 2009 Feb;85(2):310-21
Journal of leukocyte biology 2009 Feb;85(2):310-21
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Zen K, Chen CX, Chen YT, Wilton R, Liu Y
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Zen K, Chen CX, Chen YT, Wilton R, Liu Y
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2483-90
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD55/DAF in HeLa and MOLT-4 Human Cell Lines. CD55/DAF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (left panel, positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (right panel, negative staining) using Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD55/DAF in Human Colon Cancer Tissue. CD55/DAF was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 15 µg/mL Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
