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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-63072 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM170B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: GVLMFVMLQR HRQGRVISVI A Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 90%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Transmembrane Protein 170B is a Prognostic Biomarker and Associated With Immune Infiltrates in Pancreatic Adenocarcinoma.
Zhang Z, Shang J, Dai Z, Yao Y, Shi Y, Zhong D, Liang Y, Lai C, Yang Q, Feng T, Huang X
Frontiers in genetics 2022;13:848391
Frontiers in genetics 2022;13:848391
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TMEM170B in human cell line HEK 293 shows positivity in centrosome. Samples were probed using a TMEM170B Polyclonal Antibody (Product # PA5-63072).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of TMEM170B in human cell line HEK 293 using TMEM170B Polyclonal Antibody (Product # PA5-63072). Staining shows localization to centrosome.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TMEM170B in human stomach, upper using TMEM170B Polyclonal Antibody (Product # PA5-63072) shows moderate cytoplasmic and nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TMEM170B in human stomach, upper using TMEM170B Polyclonal Antibody (Product # PA5-63072) shows moderate cytoplasmic and nuclear positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 TMEM170B expression was downregulated in human pancreatic cancer cell lines and tissues. (A) TMEM170B mRNA levels were low in pancreatic cancer cell lines. (B) Real-time PCR analysis of TMEM170B mRNA expression in 38 pairs of pancreatic adenocarcinoma (PAAD) tissue and paired nontumor tissue. (C) Representative immunohistochemistry (IHC) images of TMEM170B in 38 pairs of matched tumor tissues and adjacent nontumor tissues. (D) IHC quantification was performed using ImageJ software, and TMEM170B protein expression in PAAD tissues was lower than that in nontumor tissues. (E) The subcellular localization of TMEM170B was analyzed with immunostaining (cell: scale bar, 20 mum; tissue: scale bar, 30 mum); ns: not significant. * p < 0.05; ** p < 0.01; *** p < 0.001.
