MA5-56708

antibody from Invitrogen Antibodies

Targeting: FBLL1 LOC345630

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-56708

Provider

Invitrogen Antibodies

Product name

Fibrillarin/FBLL1 Recombinant Rabbit Monoclonal Antibody (HL2588)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

HL2588

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Fibrillarin/FBLL1 using Fibrillarin/FBLL1 Recombinant Monoclonal Antibody (Product # MA5-56708). A549, H1299, and HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Fibrillarin/FBLL1 antibody diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Fibrillarin/FBLL1 using Fibrillarin/FBLL1 Recombinant Monoclonal Antibody (Product # MA5-56708). PC-12 and Rat2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Fibrillarin/FBLL1 antibody diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Fibrillarin/FBLL1 using Fibrillarin/FBLL1 Recombinant Monoclonal Antibody (Product # MA5-56708) in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Fibrillarin/FBLL1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (Product # MA5-31466) Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Fibrillarin/FBLL1 stained by Fibrillarin/FBLL1 Recombinant Monoclonal Antibody (Product # MA5-56708). Fibrillarin/FBLL1 antibody detects Fibrillarin + FBLL1 protein on Paraffin-embedded mouse liver and intestine diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Fibrillarin/FBLL1 stained by Fibrillarin/FBLL1 Recombinant Monoclonal Antibody (Product # MA5-56708). Fibrillarin/FBLL1 antibody detects Fibrillarin + FBLL1 protein on Paraffin-embedded rat liver and intestine diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Fibrillarin/FBLL1 stained by Fibrillarin/FBLL1 Recombinant Monoclonal Antibody (Product # MA5-56708). Fibrillarin/FBLL1 antibody detects Fibrillarin + FBLL1 protein on Paraffin-embedded mouse liver and intestine diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Fibrillarin/FBLL1 stained by Fibrillarin/FBLL1 Recombinant Monoclonal Antibody (Product # MA5-56708). Fibrillarin/FBLL1 antibody detects Fibrillarin + FBLL1 protein on Paraffin-embedded rat liver and intestine diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.