MA5-17609

antibody from Invitrogen Antibodies

Targeting: SNAP25 bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17609

Provider

Invitrogen Antibodies

Product name

SNAP25 Monoclonal Antibody (SP12)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-17609 targets SNAP-25 in ELISA, FACS, and WB applications and shows reactivity with Human samples. The MA5-17609 immunogen is human Synaptobrevin 1 (aa 1-118) and Synaptobrevin 2(aa 33-96).

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

SP12

Vial size

200 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase.

Verardi R, Kim JS, Ghirlando R, Banerjee A
Structure (London, England : 1993) 2017 Sep 5;25(9):1337-1347.e6

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Synaptosomal-associated protein 25 was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SNAP25 Monoclonal Antibody (SP12) (Product # MA5-17609) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing enhanced cytoplasmic and membrane localization of SNAP-25 in PC-12 cells differentiated to neuronal phenotype with NGF (200 ng/mL 5 days). Panel e represents the undifferentiated PC-12 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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