PA5-85396

antibody from Invitrogen Antibodies

Targeting: SNAP25 bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25

Antibody data

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Validation data

Product number: PA5-85396 - Provider product page
Provider: Invitrogen Antibodies
Product name: SNAP25 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (94%), Chicken (94%), Rhesus Monkey (94%), Chimpanzee (94%), Bovine (94%). Positive Control: HeLa, mouse brain, rat brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.27 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SNAP25 protein structure - PA5-85396 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SNAP25 in A) rat brain lysate using SNAP25 polyclonal antibody (Product # PA5-85396) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SNAP25 in A) mouse brain using SNAP25 polyclonal antibody (Product # PA5-85396) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SNAP25 Polyclonal Antibody detects SNAP25 protein by western blot analysis. A. 50 µg rat brain lysate/extract.12% SDS-PAGE. SNAP25 Polyclonal Antibody (Product # PA5-85396) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SNAP25 in A) HeLa cells using SNAP25 polyclonal antibody (Product # PA5-85396) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SNAP25 Polyclonal Antibody (Product # PA5-85396). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. SNAP25 Polyclonal Antibody (Product # PA5-85396) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SNAP25 Polyclonal Antibody (Product # PA5-85396). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 Polyclonal Antibody (Product # PA5-85396) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SNAP25 Polyclonal Antibody detects SNAP25 protein by western blot analysis. A. 50 µg rat brain lysate/extract.12% SDS-PAGE. SNAP25 Polyclonal Antibody (Product # PA5-85396) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.