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antibody from Invitrogen Antibodies
Targeting: SNAP25
bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-85396 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAP25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 0.27 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAP25 in A) rat brain lysate using SNAP25 polyclonal antibody (Product # PA5-85396) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAP25 in A) mouse brain using SNAP25 polyclonal antibody (Product # PA5-85396) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNAP25 Polyclonal Antibody detects SNAP25 protein by western blot analysis. A. 50 µg rat brain lysate/extract.12% SDS-PAGE. SNAP25 Polyclonal Antibody (Product # PA5-85396) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAP25 in A) HeLa cells using SNAP25 polyclonal antibody (Product # PA5-85396) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNAP25 Polyclonal Antibody (Product # PA5-85396). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. SNAP25 Polyclonal Antibody (Product # PA5-85396) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNAP25 Polyclonal Antibody (Product # PA5-85396). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 Polyclonal Antibody (Product # PA5-85396) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNAP25 Polyclonal Antibody detects SNAP25 protein by western blot analysis. A. 50 µg rat brain lysate/extract.12% SDS-PAGE. SNAP25 Polyclonal Antibody (Product # PA5-85396) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNAP25 Polyclonal Antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP25 protein stained by SNAP25 Polyclonal Antibody (Product # PA5-85396) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta-3 Tubulin Polyclonal Antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SNAP25 (Product # PA5-85396) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNAP25 Polyclonal Antibody detects SNAP25 protein at cytosol and membrane on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SNAP25 Polyclonal Antibody (Product # PA5-85396) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNAP25 Polyclonal Antibody detects SNAP25 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SNAP25 Polyclonal Antibody (Product # PA5-85396) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNAP25 Polyclonal Antibody detects SNAP25 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: SNAP25 protein stained by SNAP25 Polyclonal Antibody (Product # PA5-85396) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
